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Samples were maintained at cryogenic temperatures using a closed cycle liquid He coldhead cryostat (15 K) to minimize radiation damage and maintain an inert sample environment
Interestingly, transcriptome analysis showed that in NPCs derived from IMPA1 -mutated patients the most upregulated genes involved in cell cycle arrest were p15INK4B and p16INK4A while those linked to apoptosis were CASP4 and p16INK4A
Storing them in plastic syringes for extended periods can increase the risk of degradation and contamination
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While mild tiredness during the initial weeks of semaglutide treatment may resolve as the body adjusts, certain presentations of fatigue warrant prompt medical evaluation
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