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Samples were maintained at cryogenic temperatures using a closed cycle liquid He coldhead cryostat (15 K) to minimize radiation damage and maintain an inert sample environment

Interestingly, transcriptome analysis showed that in NPCs derived from IMPA1 -mutated patients the most upregulated genes involved in cell cycle arrest were p15INK4B and p16INK4A while those linked to apoptosis were CASP4 and p16INK4A

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While mild tiredness during the initial weeks of semaglutide treatment may resolve as the body adjusts, certain presentations of fatigue warrant prompt medical evaluation