MATERIALS AND METHODS Cell culture The human non-small cell lung cancer cell line NCI-H1299 was maintained in Dulbeccos Modified Eagles Medium (DMEM, Thermo Fisher Scientific), which was supplemented with 10% Fetal Bovine Serum (FBS, Anprotec), 2 mM glutamine (Thermo Fisher Scientific), 50 U/mL penicillin, 50 g/mL streptomycin (Gibco), 10 g/mL ciprofloxacin (Fresenius Kabi) and 2 g/mL tetracycline (Sigma-Aldrich)
Starting at a full dose is more likely to cause side effects, so treatment follows a structured escalation
More clinical trials are needed to better understand the association between B12 and brain health
May 2024: Judge Gene E
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Q: Do I need to train hard while on BPC-157