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Challenging rats with MTX evoked hepatotoxicity as manifested by elevated liver function enzymes in serum and disarrayed hepatic histopathologic features, which aligns with previous reports (Karabulut et al., 2020
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ICH Q2(R2) also notes that representative data such as chromatograms can help demonstrate specificity for assay, purity, or impurity tests [13]
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When these organs are working well, and there is sufficient water and nutrient intake, then most detox reactions pass quickly, and sometimes even go unnoticed
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