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TMT LCMS analysis Dried fractions were solubilized in 50 ml of 0.1% trifluoroacetic acid and desalted using 2-g-capacity ZipTips (Millipore) according to the manufacturers instructions

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Wilcoxon, Pearsons Chi-squared, and Fischers tests were used to identify statistical significance

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Sex differences in brain tumor glutamine metabolism reveal sex-specific vulnerabilities to treatment