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The immunoblots were visualized using ECL Western blotting substrate (Pierce Biotechnology) on a Chemidoc XRS imager (Bio Rad) or using Dylight 800-conjugated secondary antibodies (Thermo Fisher Scientific, Rockford, IL) on an Odyssey Imaging Systems (LI-COR Biosciences, Lincoln, NE)
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120 (Pt B), 24422447
Alternatively, this could be called stage 0, as there are no manifestations of disease at this point
Hydration matters too: dehydration can exacerbate fatigue during the day and paradoxically trigger wakefulness at night as your body tries to conserve water
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