Today's oral GLP-1s give 13-15% weight loss at 36 weeks (orforglipron Phase 2)
GSH depletion causes ferroptosis in RPE cells To evaluate the mechanism of cell death in GSH-depleted cells, we treated RPE cells with various cell death pathway inhibitors including ferroptosis inhibitors ferrostatin-1 (Fer-1, 8 M), liproxstatin-1 (Lip-1, 600 nM), iron chelator deferoxamine (DFO, 80 M), pan-caspase inhibitor z-VAD-fmk (30 M) to inhibit apoptosis, autophagy inhibitor 3-methyladenine (3-MA, 10 mM), and lysosomal inhibitor bafilomycin A1 (Baf-A1, 75 nM)
doi: 10.1038/srep41768 190 GrinblatGAKhanRSDineKWesselHBrownLShindlerKS
Finally, after washing with cold PBS, images were acquired via a confocal laser-scanning microscope with a 100 oil-immersion objective (LSM 700
In this reaction, deprotonated GSH bound to the G-site of the enzyme induces a nucleophilic substitution of the bond between the two oxygen atoms of the hydroperoxyl group (R-OOH), leading to the release of GSOR and an OH group that is protonated into H 2 O
A.MattinglyM