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A full risk of bias table can be found in Supplementary Table S1
Much of the observed benefit in MIC programmes plausibly comes from the programme, not the vial
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The mechanism of regain is well understood: GLP-1 medications suppress appetite while active
Freeze-thaw cycling is the specific mechanism most likely to degrade IGF-1 LR3 after it reaches you, and it is entirely under your control: a single reconstitution split into pre-planned aliquots exposes the material once, while repeatedly warming and refreezing one container exposes it as many times as you open it
There is published work on each component alone, generated in different decades by different fields with different standards of evidence, and none of those studies had the other two present
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