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2017 Cell study used 5 mg/kg intraperitoneal injection three times per week in aged mice, with treatment periods of several weeks the three-times-weekly dosing suggests the peptide maintains sufficient functional concentrations for at least 48-72 hours per dose (PMID: 28340339)

However it often reduces in supply especially when you grow older and external supplement is needed to maintain good health

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AOD9604 is not used in human medicine