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Direct attribution of unreported or underreported adverse events is not possible with available data
Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)
The native parent is a much larger (~40-kDa) protein present in human gastric juice
Increase the chlorine solution from 25 mg/L to 50 mg/L and proceed according to ANSI/AWWA 651 standards
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