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This in vitro analysis manifested the upregulation of IB expression, resulting in the inhibition of NF-B pathway, hence providing anti-inflammatory effects (Wang Fang et al., 2016)

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however, this needs to be validated in future studies. MNT also talked to Demetrios Vavvas, MD, PhD, director of the retina service at Mass Eye and Ear in Massachusetts, about this research

2.3 Determination of biochemical indicators 2.3.1 Determination of glutathione levels in heart tissue The measurements of oxidized (GSSG) and reduced glutathione (GSH) were performed in the heart homogenates with Ellmans reagent ( 2.3.2 Measurement of the H 2 S content The content of H 2 S was determined in the suspension of heart mitochondria as described previously with modifications ( 2 S will be used to represent all sulfides

Blood Collection and Biochemical Analysis At the end of the treatment period (21 days), blood samples (7ml) was obtained from the retro-orbital sinus of each animal under mild ether anaesthesia into a heparinised EDTA tubes to prevent coagulation