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Any remaining trace amounts have negligible effects
Role of PARL-PINK1-Parkin pathway in adipocyte differentiation
After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min
JG: Writing original draft, Writing review & editing, Investigation
10.1093/jn/79.1.101 38
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