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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

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Furthermore, icariin extracted from Epimedium can also function as a GPER agonist by activating GPER and subsequently increasing the expression of sirtuin 1 (Sirt1), thereby reducing the translocation and release of high-mobility group box 1 (HMGB1) and inhibiting inflammation to improve endothelial function in type 1 diabetic rats [193]