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Site-directed mutagenesis Mutations were introduced into the HPV-16 E7 plasmid construct using the QuickChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA) according to the manufacturer's instructions
1992), requiring a higher dose of the cytostatic drug 6-mercaptopurine compared to female leukemia patients (Lennard et al
Once you have mastered the technique with one peptide, it transfers directly to every other injectable in your protocol
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These viral proteins were selected based on their significant role in IAV virulence 18,19,20,21 and evasion of host immunity 22,23,24,25,26
