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References Double-blinded comparison of diphenhydramine versus lidocaine as a local anesthetic The objective of this study was to evaluate the efficacy, onset of action, duration of action, and side effects of 1% diphenhydramine compared with 1% lidocaine when used as a local anesthetic agent

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Furthermore, western blot analysis took place by initially preparing cell extracts in a lysis buffer followed by the standard method of ultrasonication