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Biochemistry, peptide
Cells were then washed, lysed in a 0.5 M Tris-HCl, pH 7.4 buffer containing 0.5% Nonidet P-40, 20 mM EDTA, 10 mM NaF, 2 mM benzamidine and a protease inhibitor cocktail (Roche), and cleared by centrifugation
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Hypothesizing that the chloro-methyl-pyrimidine core was responsible for the remaining issues, they explored replacing it with a bicyclic core, leading to Compound 80
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