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It is also essential for folate and related one-carbon metabolism, where FAD is required as a cofactor for methylenetetrahydrofolate reductase (MTHFR), a key folate-metabolizing enzyme

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Following carnitine acyl-carnitinemediated transfer of the CPT-1-generated fatty acyl-carnitines across the inner mitochondrial membrane, the fatty acyl-carnitine molecules are acted on by the inner mitochondria membrane carnitine palmitoyltransferase 2 (CPT-2 or CPT-II) regenerating the fatty acyl-CoA molecules (Figure 25-2)