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At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min

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BPC-157 availability: Single vials in 10mg (about $100) and 20mg (about $181), with bulk discounts from 5% up to 15% on larger orders