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The cell suspension was centrifuged at 14,000 g for 10 min, and 3 l of supernatant was applied to an Accucore 150 Amide HILIC HPLC column (100 2.1 mm, 2.6 m particle size) equipped with a guard cartridge (at 30 C)

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Human skin microbiome: impact of intrinsic and extrinsic factors of skin microbiota

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Smaller portions reduce nausea and make it easier to meet protein goals without feeling overly full