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The molecular mediators identified from these studies could serve as potential biomarkers

doi:10.1016/j.cmet.2018.02.011 High-Dose Vitamin C IV: Collagen Synthesis and Photoprotection Intravenous vitamin C at 10-25g achieves plasma concentrations of 13,000-20,000 umol/L 70-100x higher than maximum oral absorption of 220 umol/L

You won't experience any dangerous withdrawal effects, but the benefits you gained from treatment will slowly diminish

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Dividing the daily dose (for example, taking 100 mg twice daily rather than 200 mg once daily) may improve absorption and tolerability