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Pull back on the plunger slightly past your target mark, then push forward to the correct line to expel air bubbles

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It is produced in the liver and exists in every cell of the body

RNA extraction and qRT-PCR Total RNA was extracted from 20 freshly dissected pairs of male testes for each replicate using TRIzol (Ambion) and quantified by spectrophotometric analysis (Nanodrop, Thermo Scientific)

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