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As such, plasma which was deproteinized prior to storage at 80 o C was thawed and further diluted 1:2.5 with assay buffer to produce a final dilution of 1:5 before assaying

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In 1921, researchers isolated insulin, a peptide hormone that revolutionized diabetes treatment

The lyophilized cake should dissolve clear within a few minutes -- avoid vigorous shaking, which can denature peptides