Experiments with varying substrate concentrations revealed MichaelisMenten type kinetics with a V max of 48.1 1.4 mol min 1 mg 1 and a K m of 2.6 0.14 mM MSA (Figure 5C)
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However, the plasma IL-13 and IL-1 concentrations remained unchanged between groups ( Figures 6G, H )
Dysregulated growth factor gene expression is associated with tubulointerstitial apoptosis and renal dysfunction
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Each PCR was performed in a 20 l reaction mixture containing 2X KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems)