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As an inducer of NRF2, DMF could promote SLC7A11 and GPX4 expression, increase cellular cystine and glutathione levels, and mediate anti-oxidative stress effects

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The pipette (extracellular) solution contained 140 mM KCl, 2.6 mM CaCl 2 , 1 mM NaCl, 1.2 mM MgCl 2 , and 10 mM HEPES (pH 7.4, by KOH), while the bath (cytosolic) solution contained 130 mM NaCl, 5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES (pH 7.4, by NaOH) supplemented with desired concentrations of glucose and NMDG to bring the final osmolarity of 300 mOsm

Statistics were calculated using GraphPad Prism 8 (v.8.4.3 (686))

These results are different from our study, probably due to differences in drug dosage

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