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Story Source: Materials provided by University of Pennsylvania School of Engineering and Applied Science

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On the following day, the fluorescence-coupled secondary antibody Alexa 488 and/or 546 (1:5001:3000, Invitrogen), and 46-Diamidino-2-phenylindole dihydrochloride (Nuclei, DAPI, 1:60.000) diluted in 0.5% Triton X-100 and 0.1 M PB for 2 h at RT

This depends on how severe the deficiency was, how well they respond to treatment, and their health