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Cell surface receptor detection was performed using a mouse monoclonal (9E10) primary antibody [1:2000] to detect the c-myc tag, and a mouse raised IgG Horse Radish Peroxidase-linked secondary antibody [1:2000], both diluted in blocking solution [1 PBS containing 2% (w/v) BSA and 0.05% (w/v) Tween-20]

Regulatory agencies like the FDA require strong safety databases for drugs that will be prescribed to millions of people, and a trial of this size provides the statistical power needed to detect both efficacy signals and uncommon adverse events

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CJ: Its possible, but it doesnt always work

G.et al (2001)

Michelle Andrews, D.C