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The scoring standard for IHC staining adopted the immunoreactive score (IRS) system [15, 16], with the specific rules as follows: the staining intensity of GGCT protein was classified into four grades, where a score of 0 indicated no staining signal, 1 indicated weak positivity, 2 indicated moderate positivity, and 3 indicated strong positivity
W., Grusdat, M., Duncan, G
However, our team found that the specificity of those two compounds to xCT was relatively low, as addition of cysteine analog N-Acetyl-Cysteine (NAC), could not rescue cells from dying (16)