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Here is my method: System: Waters UPLC H-Class Column: Acquity UPLC BEH C18 1.7 um, 2.1 x 50 mm Column Temp: 60 C Run Time: 6 minutes Injection Volume: 3 uL Mobile Phase solvents: A: 0.1% Formic Acid in DI Water B: 0.1% Formic Acid in Acetonitrile Gradient: I have prepared at least two standards of GSH and one of GSSG in [50/50] HPLC Grade Methanol/DI water

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It is sometimes used to improve muscle performance in animals like racing horses, and it is also used to increase milk production in dairy cows

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