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Tsoukas MA et al
After the assessment, each bull ejaculate ( n = 9) with semen volume 4 ml, sperm cell concentration 1.0 10 9 /ml, progressive motility 75%, and sperm viability 80% were split into aliquots according to the experimental design and applied for the following experiments ( Optimization of SeNPs concentration for semen cryopreservation The basic semen extender used in this study was a Tris-based extender as distilled water supplemented with 28 mM fructose, 104 mM citric acid, 297 mM Tris buffer, 5.0% (v/v) glycerol, and 20% (v/v) egg yolk
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Very low-calorie diets (800 kcal daily) should only be undertaken under close medical supervision as part of structured programmes
During use, store below 30C or refrigerated between 2C and 8C for up to six weeks
Diabetologia 59 , 25792587 (2016)
