Future research must delineate this threshold and devise strategies, such as controlled dosing, microenvironment modulation, and combination therapies, to remain within the therapeutic window
We followed the Guiding Principles in the Care and Use of Animals of the American Physiology Society during zebrafish maintenance and experimentation
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Flow cytometric analysis The mice were anesthetized, and their livers were first perfused with calcium and magnesium-free buffer (10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [pH 7.4], 5.4 mM KCl, 120 mM NaCl, 0.5 mM EDTA, 5.6 mM glucose, and 5.0 mM NaHCO 3 ) via the inferior vena cava for 3.5 min at a rate of 7 mL/min and then with the Hanks balanced salt solution (5.4 mM KCl, 140 mM NaCl, 0.44 mM KH 2 PO 4 , 0.34 mM Na 2 HPO 4 , 1.3 mM CaCl 2 , 0.98 mM MgCl 2 6H 2 O, 5.6 mM glucose, and 4.2 mM NaHCO 3 ) containing 0.1% collagenase (032-22364
Thanks for the review