The results have been consistent across dozens of high-quality rat studies that researchers got very excited
Nrf2 activation suppresses ROS in antigen-presenting dendritic cells enhancing their capacity to interact with and promote the transformation of nave CD8 T cells into cytotoxic T lymphocytes enabling cytotoxic T-cells to eliminate virally infected cells (Kesarwani et al., 2013
You injected into a blood vessel
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Radioimmunoassay was used for measurement of insulin (Diagnostic Products, Los Angeles, CA)
The strongest, clearest evidence is biochemical: growth hormone releasing compounds like CJC-1295 raise growth hormone and IGF-1, and studies of growth hormone secretagogues confirm they can increase IGF-1, a measurable marker on the pathway (a 2017 study showing a growth hormone secretagogue raised serum IGF-1)