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Increase in intracellular Ca 2+ was monitored in cells after loading the monolayers with fura-2 AM

They are not mutually exclusive a researcher can run 5-on/2-off within an 812 week block and still take the 4-week macro-break afterward

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While balanced concentrations of Fe 2+ are essential for the survival of living organisms, iron overload is responsible for the generation of metabolically toxic ROS and phospholipid hydroxy peroxides through the Fenton reaction, ultimately leading to cell damage and death by ferroptosis [16, 17]