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The hormone-tethered receptors were expressed as fusion proteins with N-terminal IgG signal peptides to ensure proper membrane localization
Junction PCR confirmed the knock-in event in the liver, and digital PCR indicated an efficiency of approximately 1% for a single administration of Donor+Cas9, consistent with previously reported in vivo knock-in efficiencies (Fig
Ensure youre eating adequate food despite reduced appetite
They also play a role in preventing the accumulation of fats in the liver, reducing the risk of associated liver disorders
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