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At first, the surface of the cartilage becomes worn, and with the continuation of the pathological process, deep cracks related to the shedding of cartilage fragments gradually forms, causing delamination and exposure of the calcified cartilage and bone below (Sandell, 2012) (Figure 1)
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DAPI-stained nuclei with blue fluorescence were viewed under laser confocal microscope (LSM 510
Purity: 9899% by HPLC
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