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Readouts most often include glycerol release and free fatty acid quantification in the supernatant, alongside cellular ATP or viability measures to confirm that observed lipolysis reflects genuine metabolic signaling rather than cytotoxic membrane disruption

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These are given either in the muscle (intramuscularly) or under the skin (subcutaneously)

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Handling must only be performed by qualified and trained laboratory professionals in accordance with applicable regulations and institutional guidelines