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Astrup A, Rossner S, Van GL, et al

While severe cases of glutathione deficiencies are rare, mild-to-moderate cases of glutathione deficiencies often go unnoticed because the symptoms are common and minor

If using a pen with replaceable needles, attach a new needle according to the manufacturer's instructions, remove both the outer and inner needle caps, and perform a flow check/priming if required by your specific device

Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

During the course of the study, there was no change to the therapeutic regime other than the introduction of parenteral cobalamin supplementation

doi: 10.1097/QAD.0000000000002248 502 DeeksSGOverbaughJPhillipsABuchbinderS