Oocyte maturation The selected oocytes were matured in medium containing M199 medium (Gibco by Life Technologies, USA) with 0.3% bovine serum albumin (BSA), 10 IU/mL pregnant mare serum gonadotrophin (PMSG) (Intergonan, Intervet Deutschland GmbH), 10 IU/mL human chorionic gonadotrophin (hCG) (Chorulon, Intervet International BV Boxmeer-Holland), and 50 g/mL gentamycin (Sigma-Aldrich, USA), supplemented with varying concentrations of GSH (0.0 mM, 0.5 mM, 1.0 mM, and 1.5 mM)
Retrospective data on birth weight and gestational age from the control children were not available, but these children were born at term with adequate birth weights and were not hospitalised at birth
The lowest compound concentration where no bacterial growth occurs was defined as the respective MIC value
1e, yielding a GSSR product that has to change its inhibitory orientation before it can be reduced at the 1st glutathione-interaction site (Fig
Sodium hydroxide and/or hydrochloric acid may have been added during manufacture to adjust the pH (range 4.5-7.0)
23 Oral supplementation has also been studied in obese patients with or without type II diabetes to determine the impact on insulin sensitivity