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It can be traced back to the start of humanity
Spyrou and Holmgren [44] showed that inhibition of glutathione synthesis by 0.1 mM BSO was able to decrease GSH synthesis after treatment for 12 hours, but GSH-depleted cells grew as well as control 3T6 cells with no decrease in DNA synthesis
Science (New York, N.Y.) , 365(6448), 34
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Bacteriostatic Water Key Research Facts Composition: Sterile water for injection + 0.9% w/v benzyl alcohol (bacteriostatic preservative) pH: Typically 4.57.0 (most preparations 5.06.5) Preservative mechanism: Benzyl alcohol disrupts bacterial cell membrane integrity and inhibits bacterial enzyme systems Primary advantage: Multi-use capability maintains sterility across repeated needle access to the vial Shelf life after opening: Typically up to 28 days when stored correctly and accessed under sterile conditions Compatibility: Suitable for most water-soluble research peptides at neutral to mildly acidic pH Not suitable for: Peptides that require an acidic reconstitution environment use acetic acid water first for those compounds Format: Sterile vial with resealable rubber stopper for multi-use access Distributed by: Peptides Lab UK What is Bacteriostatic Water Used for in Peptide Research
The progression of the tumors was monitored by palpating every 3 days after injecting the cells until tumors were palpable, after which the tumors were measured by digital caliper every other day
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