cAMP accumulation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were diluted to the density of 6 10 5 cells/ml and INS-1(832/13) cells to 2 10 6 cells/ml in stimulation buffer [phenol-free DMEM containing 5 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) and 0.1% (w/v) BSA], and cAMP detection was carried out as described previously 48
Wait at least 30 minutes before consuming any food, beverages, or other medicines
10.1074/jbc.M113.460378 93 LeeY
Research includes onceweekly subcutaneous and oral SNACenabled formulations, with outcomes and safety characterized in controlled settings

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