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Cells were passaged at confluence with 0.05% trypsin and maintained in a 37 C incubator with humidified atmosphere of 5% CO 2 and 95% air

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92 TanakaC.AsakawaA.UshikaiM.SakoguchiT.AmitaniH.TerashiM.et al (2009)

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[2] Oral B12 supplementation generally takes longer to produce effects , with patients often noticing energy improvements within two to four weeks