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Sosne G, Kleinman HK, Springs C, Gross RH, Sung J, Kang S
Maintain refrigeration only between 36-46F (2-8C)
A recurring theme in the literature concerns nitric-oxide and growth-factor pathways
Each access must be performed with a new, sterile needle and syringe to prevent microbial contamination
After the completion of all respective treatment protocols, the media were discarded and the cells were washed twice with DPBS before being treated with 10 M BCC (1% DMSO in 100 L DPBS)
, IL-6, TNF-, and TGF) (177)
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