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For people with problems absorbing the vitamin, pregnant women, and individuals suffering from mild deficiency, a 1000 mcg dose may be recommended

H.StahlE.ErdmanR.GatalicaZ.et al (2015)

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After 6 days, cells were stained with 5 g ml 1 calcein-AM (Dojindo) at 37 C for 15 min

Room temperature exposure should be limited to brief handling or shipping periods only

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