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Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C
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To confirm the prediction accuracy of the established model, we applied the model to an independent validation cohort comprising 50 patients with CVD who had received OCCT by using urine samples (Figure S8)
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