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The resulting fractions were then re-suspended in buffer A (16 L) and analyzed on an Orbitrap Fusion mass spectrometer

The m6A modification level of total RNA was measured using EpiQuik M6A RNA Methylation Quantification Kit(Colorimetric) (Epigentek, P-9005-48) according to the manufacturers protocol, 200 ng RNA was used in assay wells

Ann N Y Acad Sci, DOI: 10.1196/annals.1320.001 Steiber, A., Kerner, J., & Hoppel, C

Neuropharmacology 110 , 396406 (2016)

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This translates to people on Zepbound losing roughly 50 pounds compared to 33 pounds on Wegovy during the 72-week trial