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A healthy liver produces an abundance of the enzyme FMO3, which neutralises the TMA by oxidising it to an odourless TMAO
Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
These terms are somewhat interpretative, lack precise definitions, vary depending upon study duration, usually do not consist of a singular cell type, and do not have exclusive pathognomic features
Wolfe, R.R., Goodenough, R.D., Burke, J.F., et al., Response of Protein urea kinetics in burn patients to different levels of protein intake, Ann
Redox considerations in female reproductive function and assisted reproduction: from molecular mechanisms to health implications
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