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When they snipped it from the protein, its transporter abilities remained intact, but it lost the ability to sense glutathione

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Plasmid cloning The murine Mytho coding sequence (1,239 bp) was amplified by cDNA obtained from skeletal muscles of tumor-bearing mice and cloned in the p3XFlag-Myc-CMV vector (6.4 kb) (Addgene) using KOD Hot Start DNA polymerase (Merck Millipore) and the following primers with sticky ends: Fw 5-AAAGATCTACTGGACCTGGAGGTGGT-3 and Rw compl

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