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The cell pellet was resuspended in ice-cold 100 mM Tris, pH 7.6, centrifuged again, and finally resuspended in 1 mL of the same buffer
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Only the L-isomer of carnitine is biologically active (1)
If any of those sound familiar, especially if youre vegetarian, over 50, or on long-term acid reflux or diabetes medication, a B12 shot is a low-risk way to find out if low B12 is part of the picture
doi: 10.1038/s41418-021-00859-z 23 ChenXYuCKangRKroemerGTangD
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