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Calcium imaging Brain slices were prepared from adult NMRI male mice (aged 1828 days)

Levothyroxine dosing is influenced by body weight, with typical maintenance doses ranging from 1.6-1.8 mcg/kg/day based on ideal or lean body weight (using actual body weight in obesity may lead to overreplacement)

Then, 1.5 mL of extraction buffer (80% methanol with 0.1% formic acid, v/v) was added, with vortexing for sample resuspension

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This sheds lights on a new family of antidiabetic medications, the dipeptidyl peptidase 4 inhibitors (DPP4i) or gliptins, which target incretin hormones