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As a sixth example, the amino group of the N-terminal amino acid has a predominant positive net charge below pH~8.0, thus for cation exchange the pH elution gradient with a solvent comprising an organic modifier could begin below pH 8.0 to elute the target GLP-1 moiety which is extended with a desired acyl group and end the gradient above pH 8.0 thereby subsequently removing the undesired non-extended form
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