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For LoS, the perturbed peripheral directions were obtained by connecting the base of the true LoS direction on the nostril plane with points that radially lie 1 mm away from a point on the LoS

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A number of factors contribute: Different manufacturers use different stabilization/packaging processes (e.g., presence of excipients, vacuum sealing, desiccants)

Using forceps, the stimulus was applied to the rats hind limb by momentarily pinching the areas innervated by the tibial, fibular, and sural nerves