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For M1 polarization, RAW264.7 cells were stimulated with LPS (100 ng mL 1 , Sigma-Aldrich, USA) and IFN- (20 ng mL 1 , ABclonal, China) for 24 h

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The cells were plated on collagen-coated 96-well plates at the density of 2 10 4 per well in 0.1 mL DMEM, treated subsequently as described above

Research has demonstrated involvement of the NO system, growth factor modulation (including VEGF, EGF), and FAK-paxillin signaling pathways